This list was inspired by Robert’s Cryobiology and Electron Microscopy reading list.
When I started doing research again, Alex was super great and sent me a slew of papers to catch me up to the latest developments in synthetic biology relating to Cas9.
A while ago, I made a post with some links introducing Cas9, but after seeing Robert’s list, I feel I should post the entire list of Alex’s recommendations.
So here it is!
Introduction to Cas9:
Development and Applications of CRISPR-Cas9 for Genome Engineering
This is a very well written and easily digestible summary covering events from the discovery of Cas9 to recent applications in biological engineering.
A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
The key paper that uncovered Cas9’s behavior and opened the door for engineering with Cas9. It’s a little dense for those not in the field.
Applications to mammals and humans:
Multiplex Genome Engineering Using CRISPR/Cas Systems
Native Cas9 cleaves DNA in bacterial cells. This paper shows that Cas9 can be used for target cleaving in human and mouse cells using a split Cas9 system.
RNA-Guided Human Genome Engineering via Cas9
Native Cas9 uses two guide RNAs to recognize what target to cleave. This paper shows that the two guide RNAs can be combined into one for easier engineering.
Related to my research:
A Split-Cas9 Architecture for Inducible Genome Editing and Transcription Modulation
The paper describes how Cas9 can be split and and modified to reassemble in the cell in the presence a specific molecule.
Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA
This is one of a few papers describing the structure of Cas9. The structural data from this paper is submitted to the Protein Data Bank as ID:4OO8.
Small Molecule–Triggered Cas9 Protein with Improved Genome-Editing Specificity
Cas9 can be fused with other domains that it only activates in the present of particular molecule
Highly Efficient Cas9-Mediated Transcriptional Programming
Cas9 can be fused to transcription activators to create a custom genetic ON switch
Check these papers out if you want a better understand of why Cas9 is so hot in synthetic biology right now. They’re a good read and present some really interesting possibilities.