Thermocyclers makes transformations a lot easier by eliminating heat shocking the bacteria in a warm water bath.

I was hesitant to use the ProFlex PCR machine for transformations because its minimum temperature is 0°C. Previous transformations I’ve done via  thermocyclers always started the reaction at -4°C. I wasn’t sure if the 0°C would suffice. The manual protocol just says keep the samples on ice until ready for heat shock. I was searched the web for a transformation protocol for thermocyclers, but didn’t find anything substantial.

So I just did transformations with the ProFlex PCR machine to see what happens.

Turns out 0°C totally works! I’ve now done numerous transformations via the ProFlec PCR machine and they’ve worked. Having the lid heated vs unheated didn’t seem to make too much of a difference. I eventually opted for all unheated lid (just in case ambient heat was affecting my cells). I don’t have any data on its transformation efficiency versus a traditional method or versus a -4°C capable thermocycler. All I know is it worked for me.

Here’s the protocol:

5 min at 0°C #ramp down to 0°C, then load in the samples
30 min at 0°C
30 sec at 45°C
5 min at 0°C

Try it out! Let me know!

Don’t forget to put the PCR tubes in a cold block while pipetting!

We went to Wagamama the other night, and to my surprise, they removed chili men from their menu!

It was my favorite dish there! :'(

Technically Wagamama released their chili sauce recipe, so I could make my own, but visiting Wagamama will never be the same again. :(

Our miracle waiter informed me that they had a last batch of sauce left, so I could savor it one last time!

chilimien

Mhmmm…. the last of the chili men.

I was visiting Camden, Maine this weekend and found an adorable little local game shop called Knowledge Vault. They were hosting pre-release events for the new Magic the Gathering set so of course I had to play.

I got really lucky and opened some great white cards, so I went mono white for my deck.

sword of the animist

Sword of the Animist turned out to be an unexpectedly awesome. I was often stuck on two mana, and equipping the Sword on a low cost creature like Anointer of Champions helped me get a lot more lands.

archangel of tithes

Archangel of Tithes was brutal for my opponents. Whenever it landed on the battlefield, I knew the fight was in my favor.

liliana

I ended placing top 8 in the tournament! I was super excited ’cause it was my first time making it so far. From my prize packs, I pulled a Liliana! Liliana is my favorite character in the Magic the Gathering universe, so I was elated!

Overall, I had a great time. Knowledge Vault was super cool. The regulars seem very friendly and I think everyone had fun. Definitely check it out if you’re near the area.

Here’s my deck list:

Creatures
1x Sentinel of the Eternal Watch
1x Patron of the Valiant
1x Guardians of Meletis
1x Anointer of Champions
1x Topan Freeblade
1x Akroan Jailor
1x War Oracle
1x Auramancer
1x Stalwart Aven
2x Kytheon’s Irregulars
1x Runed Servitor
1x Ampryn Tactician
1x Archangel of Tithes

Other
3x Suppresion Bonds
1x Alchemist’s Vial
1x Sword of the Animist
1x Celestial Flare
1x Grasp of the Hieromancer
1x Gideon’s Phalanx

Lands

18x Plains